Journal: BMC Molecular and Cell Biology

Article Title: ILK supports RhoA/ROCK-mediated contractility of human intestinal epithelial crypt cells by inducing the fibrillogenesis of endogenous soluble fibronectin during the spreading process

doi: 10.1186/s12860-020-00259-0

Figure Lengend Snippet: Adhesion to exogenous FN coating supports the restoration of the contractile phenotype in ILK-depleted HIEC. a IF microscopy images representative of the actin cytoskeleton (detected with TRITC-phalloidin; in red) and vinculin-positive ECM adhesion structures (anti-vinculin antibody; green) stained in siCNS and siILK cells grown for 24 h on serum-pretreated, FN-coated (FN; 3 μg/cm 2 ) or Col-I-coated (Col-I;10 μg/cm 2 ) glass coverslips. b Histograms of the average surface spreading area (μm 2 ) measured numerically (Metamorph Imaging System software) on cells cultured under the same conditions as in (a). Results are expressed as the mean ± SEM ( n = 3; > 50 cells have been analyzed for each condition). *** p < 0.001 in a one-way ANOVA using Tukey’s correction for multiple group comparisons. Scale bar in (a) 25 μm

Article Snippet: Scale bar in (b, c, and d): 15 μm Fig. 2 Adhesion to exogenous FN coating supports the restoration of the contractile phenotype in ILK-depleted HIEC. a IF microscopy images representative of the actin cytoskeleton (detected with TRITC-phalloidin; in red) and vinculin-positive ECM adhesion structures (anti-vinculin antibody; green) stained in siCNS and siILK cells grown for 24 h on serum-pretreated, FN-coated (FN; 3 μg/cm ) or Col-I-coated (Col-I;10 μg/cm ) glass coverslips. b Histograms of the average surface spreading area (μm ) measured numerically (Metamorph Imaging System software) on cells cultured under the same conditions as in (a).

Techniques: Microscopy, Staining, Imaging, Software, Cell Culture